- ¥315
- 江蓝纯
- ER0151
- 2025年07月15日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 英文名：
BsuRI (HaeIII)
- 库存：
99
- 供应商：
江蓝纯
- 规格：
3000units
| 5' | G | G ↓ | C | C | 3' | ||||
| 3' | C | C ↑ | G | G | 5' |
Thermo Scientific BsuRI (HaeIII) restriction enzyme recognizes GG^CC sites and cuts best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI). See for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Inverse PCR For use with Snyder mTn-lacZ/LEU2 based mutagenesis
Inverse PCR I. Genomic DNA Prep • from 5 ml culture, resuspend in 50 µl TE II. Digestions genomic DNA 5 µl 10x of appropriate
the following DNA dilution, and aliquot 35 μl into ten 1.5 ml microcentrifuge tubes: DNA 100 μg 10X TM buffer 35 μlsterile ddH2 O q.s. Final Volume 350 μl 2. To determine the optimal sonication conditions, sonicate the DNA samples in five of the tubes
for sonicated fragments and four tubes for nebulized fragments. 5a. Incubate at 70℃ for 10 minutes, and place the samples in an ice-water bath. 6a. Add the following reagents for the kinase reaction and incubate at 37 ℃ for 10-30 minutes: 10 mM rATP 1 μl 10 X
技术资料暂无技术资料 索取技术资料
