AGC1, ARALAR1, Solute Carrier Family 25 Member 12, Mitochondrial Aspartate Glutamate Carrier 1, Calcium Binding Mitochondrial Carrier Superfamily Member Aralar1
Cat #: ANT-112
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IFC, IH, IP
Reactivity: H, M, R
Anti-SLC25A12Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
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Anti-SLC25A12 (#ANT-112) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize AGC1 from human, mouse, and rat samples.
Applications
Specifications
Scientific Background
Related Products
Western blot
Western blot analysis of rat skeletal muscle lysate (lanes 1 and 4, 1:600), mouse brain membrane (lanes 2 and 5) and human OVCAR3 cell line lysate (lanes 3 and 6): 1-3. Anti-SLC25A12 antibody (#ANT-112), (1:200).
4-6. Anti-SLC25A12 antibody, preincubated with the control peptide antigen.
Immunogen
Peptide (C)KDKSKSGMISGLD, corresponding to amino acid residues 169-181 of mouse SLC25A12 (Accession Q8BH59). Intracellular, N-terminus.
HomologyRat, human – identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background
SLC25 is a large family of nuclear-encoded transporters embedded in the inner mitochondrial membrane and in a few cases other organelle membranes.
SLC2512A is a gene the aspartate/glutamate carrier1 (AGC1). AGC1 possesses six transmembrane alpha helices and N- and C-termini on the cytosolic side of the membrane1.
AGC1 catalyzes an exchange between intra-mitochondrial aspartate and cytosolic glutamate, an important step in urea synthesis. As a component of the malate–aspartate shuttle (MAS), AGC1 also has a role in the transfer of NADH, carrying reducing equivalents from the cytosol into the mitochondrial matrix. AGC1 is expressed predominantly in heart and skeletal muscle, with weaker expression in brain and kidney. AGC1 is the main AGC isoform present in the adult brain, and it is expressed mainly in neurons. As part of the MAS, AGC1 has a role in the physiology of pancreatic β-cells by influencing glucose-induced activation of mitochondrial metabolism and insulin secretion and it is the only AGC isoform expressed in pancreatic islets and β-cells.
AGC1 has several Ca2+-binding EF-hand motifs located on its long, hydrophilic amino-terminus which extends into the intermembrane space and its activity is strongly regulated by extra-mitochondrial Ca2+. Dysregulation of cellular Ca2+ homeostasis mediated by AGC1 has been implicated in the pathogenesis of autistic spectrum disorder2.
References
Palmieri, F. (2013) Mol. Aspects Med.34, 465.
Napolioni, V. et al. (2011) Mol. Neurobiol. 44, 83.