Anti-P2X1 Receptor (extracellu

Anti-P2X1 Receptor (extracellular)-FITC

Purinergic Receptor P2X1, P2RX1
Cat #: APR-022-F
Sizes: 50 µl
Source: Rabbit
Type: Polyclonal
Applications: FC
May also work in: IC, LCI
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2X1 Receptor (extracellular) (#APR-022) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize P2X1 receptor from rat, mouse, and human samples.

Alomone Labs is pleased to offer a new version of this antibody that is directly conjugated to fluorescein isothiocyanate (FITC). Anti-P2X1 Receptor (extracellular)-FITC antibody (#APR-022-F) can be used in immunofluorescent applications such as direct live cell flow cytometry.

• Applications
• Specifications
• Scientific Background
• Related Products

Direct flow cytometry

Direct flow cytometry of live intact human MEG-01 megakaryoblastic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC
^___ Cells + Anti-P2X1 Receptor (extracellular)-FITC antibody (#APR-022-F), (5 µg antibody/0.5x10⁶ cells).

Direct flow cytometry of live intact mouse M1 myeloid leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC
^___ Cells + Anti-P2X1 Receptor (extracellular)-FITC antibody (#APR-022-F), 5µg.
Immunogen
Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575). Extracellular loop.

HomologyRat, mouse - 11/14 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
LabelFluorescein isothiocyanate (FITC).
Standard quality control of each lotWestern blot analysis (unlabeled antibody), and direct flow cytometry (labeled antibody).
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl double distilled water (DDW).
Antibody concentration after reconstitution1 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl DDW.
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific backgroundThe P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.

The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.

Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned^1-3. All P2X receptor subtypes share the same structure of intracellular N- and C-termini two membrane-spanning domains and a large extracellular loop.

All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex^4-9.

The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous systems, while the P2X7 receptor is predominantly found in cells of the immune system⁴. The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis^3,4,10,11.
References

1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego.
4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152.
7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378.
8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107.
9. Kim, M. et al. (2001) EMBO J. 20, 6347.
10. Burnstock, G. (2001) Trends Pharmacol. Sci. 22, 182.
11. Oury, C. et al. (2003) Blood 101, 3969.