一,质粒描述:
启动子: Polyhedrin
复制子: ColE1 ori
终止子: polyhedrin 3
质粒分类: 昆虫细胞杆状病毒组成型表达质粒
质粒大小: 9032bp
原核抗性: 氨苄青霉素Ampicillin
克隆菌株: DH5α
培养条件: 37℃,有氧,LB
表达宿主: 昆虫细胞
诱导方式: 组成性表达,无需诱导
5'测序引物: Polyhedrin-F:AAATGATAACCATCTCGC
3'测序引物: Polyhedrin-R:GTCCAAGTTTCCCTG
pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.