For Research Use Only in the United States. Not for Use in Diagnostic Procedures
The MicroVue TRAP5b Assay is an immunocapture enzyme assay for the determination of tartrate-resistant acid phosphatase isoform 5b (TRAcP 5b) in human serum and plasma.
Features & Benefits
Feature
Benefit
Product Specifications
Format
ELISA
Test/kit:
96 wells/40 (in duplicate)
Sample type:
Plasma and serum
About the Assay
Format
96 well assay plate
Species Reactivity
Reactive with human derived TRAP5b only. No reactivity detected with Cow, Chicken, Goat, Mouse, Rabbit, Rat, Sheep, Guinea Pig, Baboon, Cynomolgus Monkey, Rhesus Macaque
Assay Steps
Add 100 µL of Sample Diluent Add 50 µL of Calibrator, Controls and Specimens Seal the microplate Incubate for 60 minutes at room temperature on a microplate shaker Wash the microplate wells three times with Wash Buffer Add 100 µL of Substrate Solution Seal the microplate Incubate for 60 minutes at 37°C Add 50 µL of Stop Solution Read OD at 405 nm Data Reduction Quadratic