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- 详细信息
- 技术资料
- 保存条件:
2-8℃
- 保质期:
6个月
- 英文名:
Intact Parathyroid Hormone (1-84)
- 库存:
大量
- 供应商:
上海信裕生物科技有限公司
- 规格:
1 kit
MicroVue Intact PTH EIA
Features & Benefits
| Feature | Benefit |
|---|
Product Specifications
| Format | ELISA |
| Test/kit | 96 wells/plate |
| Sample type | Serum |
Performance Characteristics
Method: Immuno-capture
Analyte: 25-OH Vitamin D2 & D3
Specimen: Serum
Specimen Volume: 50 µL
Limit of Detection: 1.5 ng/mL
Dynamic Range: 0-133 ng/mL
Precision CVs within-run: 2.7%-5.7%
Precision CVs between-run: 4.3%-4.7%
Incubation Time: 3 Hours
About the Assay
Assay Steps
- Add 50 µL of Standard, Control or sample
- Add 150 µL of the Assay Buffer
- Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 2 hours
- Wash 3 times with 1X Wash Buffer
- Add 200 µL of working HRP conjugate
- Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 30 minutes
- Wash 3 times with 1X Wash Buffer
- Add 100 µL of the TMB Substrate
- With appropriate cover to avoid light exposure, place the microplate(s) on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 15 minutes
- Add 100 µL of Stop Solution and read at 450 nm
- Data Reduction: 4 parameter logistics, or B/B0% interpolation
Ordering Information
| Catalog Number | Description |
|---|---|
| 8046 | 96 Wells/Plate |
Product Documentation
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MicroVue Intact PTH EIA
$443









