- $410
- BioPorto
- 丹麦
- HYB 050-046
- 2025年07月12日
- ELISA, WB, IHC
- Human
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
冻干粉或液体
- 保存条件：
-20ºC
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
1年
- 抗原来源：
见官方网站
- 目录编号：
HYB 050-046
- 级别：
见官方网站
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 宿主：
Human
- 应用范围：
ELISA, WB, IHC
- 浓度：
1 mg/ml
- 靶点：
见官方网站
- 抗体英文名：
Anti-Complement component C2 (human)
- 抗体名：
Anti-Complement component C2 (human)
- 规格：
200ug
Anti-Complement component C2 (human)
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- HYB 050-046
- Product name
- Anti-Complement component C2 (human)
- Description
- Mouse monoclonal antibody
- Specificity
- HYB 050-04 is specific for C2 from human plasma/serum. Cross-reactivity to C2 from other species has not been tested.
- Immunogen
- Complement component C2 isolated from human plasma (1)
- Application
- ELISA, WB, IHC
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
- IgG2a/k
- Gene ID
- 717
- Clone number
- 5C3
- Epitope
- Epitope specificity differs from that of HYB 050-05 and HYB 050-08 as determined by inhibition ELISA.
- Content
- Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
- Complement component C2 is a component of the classical complement pathway and is involved in activation of C3 and C5. C2 consists of a single polypeptide chain with a molecular mass of 102 kDa.
- Application 1
-
ELISA
HYB 050-04 reacts with human C2 in both native and denatured forms. In ELISA, strong reaction is seen with C2 directly coated onto the microtiter well. (1) - Application 2
-
WB
In Western blotting after SDS-PAGE, HYB 050-04 reacts with C2 and a subfraction of C2 believed to be C2b, both under reducing and non-reducing conditions. (1)
HYB 050-04 was used in WB. Please consult Proteinatlas.org
WB with HYB 050-04:
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Antibody dilution: 1:500 - Application 3
-
IHC
HYB 050-04 was used in IHC. Please consult Proteinatlas.org
Immunohistochemical staining of human kidney with HYB 050-04 shows strong positivity in extracellular material.
Antibody dilution: 1:4000
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文献和实验Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Human complement component C2 is a critical factor of the classical complement pathway. Here we provide a method for the production of recombinant human C2 (rhC2) protein for research purposes. The human complement component C2 (hC
Molecular Analysis of Complement Component C4 Gene Copy Number
Classical, alternative, or lectin pathways may activate the complement system cascade. The classical pathway includes the C4 protein and functions in the prevention of immune complex precipitation and in clearance of immune complexes
Purification and Characterization of Human and Mouse Complement C3
Complement component C3 is the most abundant complement protein in plasma, central to all three complement activation pathways and essential to complement amplification. Thus, it is one of the most extensively studied complement proteins
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