CSI 005-22 is highly specific for bovine fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin).
Immunogen
Bovine corneal endothelial cells on beads
Application
ELISA, WB, IHC-F, IHC-P, IP
Species reactivity (positive)
Bovine
Species reactivity (negative)
Human
Subclass
IgG1/k
Gene ID
280794
Clone number
A22
Epitope
Not determined
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).
Application 1
ELISA
CSI 005-22 can be used in ELISA (1,2,3)
Application 2
WB
CSI 005-22 can be used in immunoblotting
Application 3
IHC
CSI 005-22 can be used i in immunostaining of frozen PLP-fixed sections of bovine tissues.