GC Conditions: Column: RTX-1 30m x0.25mm x0.2µm Carrier Gas: helium Linear Velocity: 20cm/sec Make-up Gas: nitrogen Flow Rate: 40ml/min Split Ratio: 100:1 Vent Flow: 70ml/min Oven Initial: 325°C Program Rate: Isothermal Detector: FID, 350 °C Injector: 350°C GC elution order: Brassicasterol, Campesterol, Stigmasterol, beta-Sitosterol This qualitative mixture contains four major plant sterols isolated from natural sources and is ideal for use as a standard with gas chromatography, 1 mass spectrometry, and high performance liquid chromatography.