- $595
- BioPorto
- 丹麦
- ABS 012-356
- 2025年07月14日
- ELISA
- Human
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
冻干粉或液体
- 保存条件：
-20ºC
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
1年
- 抗原来源：
见官方网站
- 目录编号：
ABS 012-356
- 级别：
见官方网站
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 宿主：
Human
- 应用范围：
ELISA
- 浓度：
1 mg/ml
- 靶点：
见官方网站
- 抗体英文名：
Anti-Exendin-4
- 抗体名：
Anti-Exendin-4
- 规格：
200ug
Anti-Exendin-4
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- ABS 012-356
- Product name
- Anti-Exendin-4
- Description
-
Mouse monoclonal antibody
- Specificity
-
Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.
- Immunogen
-
Synthetic exendin-4
- Application
- ELISA
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Gene ID
- 2740
- Clone number
- 35
- Epitope
-
The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exendin (9-39)amide.
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Exendin-4 is a 39 amino acid peptide found in venom from the Gila monster Heloderma suspectum. It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion.
- Application 1
-
ELISA
ABS 012-35 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between ABS 012-35 and exendin-4 is disrupted by 4.5 M MgCl2.
ABS 012-35 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay (1).
By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. ABS 033-10 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.
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