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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
见官方网站
- 亚型:
见官方网站
- 形态:
冻干粉或液体
- 保存条件:
-20ºC
- 克隆性:
多克隆
- 标记物:
见官方网站
- 适应物种:
见官方网站
- 保质期:
1年
- 抗原来源:
见官方网站
- 目录编号:
HYB 162-022
- 级别:
见官方网站
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA, WB
- 浓度:
1 mg/ml
- 靶点:
见官方网站
- 抗体英文名:
Anti-Complement component C4 (human)
- 抗体名:
Anti-Complement component C4 (human)
- 规格:
200ug
Anti-Complement component C4 (human)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- HYB 162-022
- Product name
- Anti-Complement component C4 (human)
- Description
- Mouse monoclonal antibody
- Specificity
- HYB 162-02 is specific for human C4.
- Immunogen
- C4 isolated from human plasma
- Application
- ELISA, WB
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
- IgG1/k
- Gene ID
- 720
- Clone number
- 25.3F4
- Epitope
- Epitope specificity differs from that of HYB 162-04, as determined by inhibition ELISA.
- Content
- Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
- C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b. Together C2a and C4b forms the classical C3 convertase. The molecular mass of C4 is 204 kDa and it consists of 3 domains: α (97kDa), β (75 kDa) and g (33 kDa).
- Application 1
-
ELISA
HYB 162-02 reacts with C4. A strong reaction is seen with normal human serum when tested in sandwich ELISA in combination with a polyclonal antibody against C4 (e.g. DAKO A065). No reaction is seen with C4-depleted serum (1). - Application 2
-
WB
In Western blotting after SDS-PAGE, HYB 162-02 reacts with C4 in both reduced and non-reduced forms (2,3).
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文献和实验Molecular Analysis of Complement Component C4 Gene Copy Number
Classical, alternative, or lectin pathways may activate the complement system cascade. The classical pathway includes the C4 protein and functions in the prevention of immune complex precipitation and in clearance of immune complexes
Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Human complement component C2 is a critical factor of the classical complement pathway. Here we provide a method for the production of recombinant human C2 (rhC2) protein for research purposes. The human complement component C2 (hC
Complement C4 Protein and DNA Typing Methods
Complement C4 is the only component coded for by two nearly identical isotypic genes, C4A and C4B. The C4 genes are located tandemly arranged each with a steroid 21-hydroxylase (CYP21) gene at its 3′ end, together with the genes for C2
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