- $410
- BioPorto
- 丹麦
- CSI 005-321
- 2025年07月14日
- ELISA, WB, IHC-F, IHC-P, IP
- Bovine, human
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
冻干粉或液体
- 保存条件：
-20ºC
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
1年
- 抗原来源：
见官方网站
- 目录编号：
CSI 005-321
- 级别：
见官方网站
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 宿主：
Bovine, human
- 应用范围：
ELISA, WB, IHC-F, IHC-P, IP
- 浓度：
1 mg/ml
- 靶点：
见官方网站
- 抗体英文名：
Anti-Fibronectin (bovine, human)
- 抗体名：
Anti-Fibronectin (bovine, human)
- 规格：
200ug
Anti-Fibronectin (bovine, human)
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- CSI 005-321
- Product name
- Anti-Fibronectin (bovine, human)
- Description
-
Mouse monoclonal antibody
- Specificity
-
CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain.
- Immunogen
-
Bovine corneal endothelial cells
- Application
- ELISA, WB, IHC-F, IHC-P, IP
- Species reactivity (positive)
- Bovine, human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Gene ID
- 280794 (2335)
- Clone number
- A32
- Epitope
-
Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of CSI 005-35
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).
- Application 1
-
ELISA
CSI 005-32 can be used in ELISA. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody CSI 005-35. (1, 2, 3, 4) - Application 2
-
WB
In Western blotting dilution guideline of 1/100 has proved successful (1). - Application 3
-
IHC
CSI 005-32 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. - Application 4
-
IP
CSI 005-32 can be used in immunoprecipitation.
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文献和实验Purification of Fibronectin from Human Plasma
Fibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogen-esis
Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
a simple ELISA method to detect human anti-antibody response.
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