- $410
- BioPorto
- 丹麦
- HYB 190-011
- 2025年07月13日
- ELISA, WB, IHC
- Human, bovine, Torpedo marmorata
- 见官方网站
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
见官方网站
- 亚型：
见官方网站
- 形态：
冻干粉或液体
- 保存条件：
-20ºC
- 克隆性：
多克隆
- 标记物：
见官方网站
- 适应物种：
见官方网站
- 保质期：
1年
- 抗原来源：
见官方网站
- 目录编号：
HYB 190-011
- 级别：
见官方网站
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 宿主：
Human, bovine, Torpedo marmorata
- 应用范围：
ELISA, WB, IHC
- 浓度：
1 mg/ml
- 靶点：
见官方网站
- 抗体英文名：
Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
- 抗体名：
Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
- 规格：
200ug
Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- HYB 190-011
- Product name
- Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYB 190-01 is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE.
- Immunogen
-
C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme.
- Application
- ELISA, WB, IHC
- Species reactivity (positive)
- Human, bovine, Torpedo marmorata
- Species reactivity (negative)
- Electric eel, human
- Subclass
-
IgG1/l
- Gene ID
- 43, 540446
- Clone number
- 4E11
- Epitope
-
C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1).
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.
Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.- Application 1
-
ELISACan be used in ELISA on amniotic fluid for the diagnosis of neural tube defects.HYB 190-01 is well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann’s reaction) (1).
- Application 2
- WB
In Western blotting and dot blotting HYB 190-01 reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE..In Western blotting a dilution guideline of 1/75 has proved successful (1).
- Application 3
- IHC
HYB 190-01 can be uesd in Immunohistochemistry.
Human placenta stained with HYB 190-01
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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
Mammalian Brain Acetylcholinesterase
Acetylcholinesterase (acetylcholine acetylhydrolase, AChE: EC 3.1.1.7) has been the focus of much attention since it was first suggested that it plays an important role in the rapid destruction of acetylcholine (ACh) in a living organ (Dale, 1914
在动物的神经系统中，神经细胞集聚形成神经作用支配中心的部分。在无脊椎动物，一般把头神经节称脑。脊椎动物的脑接于脊髓前方，与脊髓同在身体的背正中部，是由该部外胚层的陷入所生成的神经管发生。随着它的复杂化，脑为脑膜所直接包裹，特别是由软骨或骨形成的脑颅来保护。在原索动物中只有头索类有脊椎动物型的脑，由前脑和后脑构成，但与脊髓界限不清，仅能由散在后脑背部的 Joseph 细胞的存在与脊髓相区别。圆口类以上的脑膨起，与脊髓有明显的区别。并可区分与前脑
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