This product consists of 2 antibodies that in combination can be used to measure Anti-yENaC (y−subunit epithelial sodium channel, neoepitope). CAM 003-07 as capture antibody (1-4 µg/mL) and CAM 005-02B as biotinylated detection antibody (0.05 - 0.2 µg/mL) form a sandwich ELISA pair for measuring Anti-yENaC.
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
Application
ELISA
Target
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the t-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.
Application 1
ELISA
CAM 003-07 was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order
to detect the peptide from the inhibitory tract (AA 138-131).
The calibration curve of a sandwich assay for Anti-yENaC using CAM 003-07 as the capture antibody and CAM 005-02B as the biotinylated detection antibody.