This product consists of 2 antibodies that in combination can be used to measure Total Human NGAL. ABS 038-14 (as capture antibody) and ABS 038-15B (as biotinylated detection antibody) form a sandwich ELISA pair for measuring Total Human NGAL. Tthe assay reacts equally with NGAL monomer and homodimer on a mass basis.
ABS 038-14-02
495 €
ABS 038-15B-005
380 €
Total
875 €
Matched Pair Price
785 €
Immunogen
Recombinant human NGAL
Application
ELISA
Content
200 µL capture antibody and 50 µl detection antibody. 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Target
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.
Application 1
ELISA ABS 038-14 (as capture antibody) forms a sandwich ELISA pair with ABS 038-15B (as biotinylated detection antibody) for measuring total human NGAL.
The figure illustrates the calibration curve of a sandwich assay with ABS 038-14 as the capture antibody and ABS 038-15B as the biotinylated detection antibody.