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- 供应商:
江蓝纯
- 规格:
22次
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P2078 | ChIP Assay Kit | 22次 | 1396.00元 |
ChIP Assay Kit即Chromatin Immunoprecipitation(ChIP)Assay Kit,也称染色质免疫沉淀检测试剂盒或ChIP检测试剂盒,用于通过免疫沉淀来沉淀和目标蛋白结合的染色质片段,后通过PCR或Southern等方法来检测沉淀的染色质片段的试剂盒。通常用于检测特定的转录因子或组蛋白等基因组DNA结合蛋白是否和预期的特定基因组DNA序列在同一复合物中。
通过ChIP检测可以获得在体的(In Vivo)目标蛋白和预期基因组DNA片段是否在同一复合物中的结论。EMSA,也称gel shift获得的结果是体外的(In Vitro)目标蛋白和预期基因组DNA片段的结合结果,可以推断细胞内也发生类似的结合,但并不代表该情况在细胞内也真实发生。而ChIP的检测结果则可明确说明这种结合在细胞内是真实发生的。
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文献和实验were washed with PBS and resuspended in ChIP lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.0) add protease inhibitors (inhibitors: 1mM PMSF, 1microgram/ml aprotinin and 1microgram/ml pepstatin A).. After incubated 10 minutes on ice, cells were sonicated
method. The Active Motif ChIP-IT Kit comes with all the reagents needed for 25 reactions of the ChIP assay. In addition, it provides a detailed manual which saves time for protocol optimization. The protocol in the manual is easy to follow
Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
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