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-20℃
6个月
pBBR1MCS-6
大量
钰博生物
1ug/5ug
出品人: | Ybscience |
---|---|
载体名称: | pBBR1MCS-6 |
质粒类型: | 广宿主载体;克隆载体;原核表达载体 |
高拷贝/低拷贝: | -- |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | lac |
载体大小: | 5859 bp |
5' 测序引物及序列: | M13 Forward 5’TGTAAAACGACGGCCAGT 3’ |
3' 测序引物及序列: | M13 Reverse 5’CAGGAAACAGCTATGAC 3’ |
载体标签: | -- |
载体抗性: | 链霉素 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 多个菌属 |
备注: | 可用蓝白斑筛选法 |
组成型/诱导型: | 诱导型 |
LOCUS KC698875 5859 bp DNA circular SYN 26-AUG-2013DEFINITION Cloning vector pBBR1MCS-6, complete sequence.ACCESSION KC698875VERSION KC698875.1 GI:531997060 AUTHORS Meng,J., Wang,H., Liu,X., Lin,J., Pang,X. and Lin,J. TITLE Construction of small plasmid vectors for use in genetic improvement of the extremely acidophilic Acidithiobacillus caldus JOURNAL Submitted (27-FEB-2013) State Key Laboratory of Microbial Technology, Shandong University, Shanda Nanlu 27#, Jinan, Shandong 250100, ChinaFEATURES Location/Qualifiers source 1..5859 /organism="Cloning vector pBBR1MCS-6" /mol_type="other DNA" /db_xref="taxon:1387373" gene complement(join(5660..5859,1..799)) /gene="mob" CDS complement(join(5660..5859,1..799)) /gene="mob" /note="required for plasmid mobilization" /codon_start=1 /transl_table=11 /product="Mob" /protein_id="AGT62526.1" /db_xref="GI:531997062" /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLG" gene 1793..2455 /gene="rep" CDS 1793..2455 /gene="rep" /note="required for pBBR1 derivative plasmid replication" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AGT62525.1" /db_xref="GI:531997061" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" gene complement(3013..3378) /gene="lacZ" CDS complement(3013..3378) /gene="lacZ" /note="beta-galactosidase; alpha peptide of LacZ" /codon_start=1 /transl_table=11 /product="LacZ" /protein_id="AGT62527.1" /db_xref="GI:531997063" /translation="MTMITPSAQLTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPL VLERPPPRWSSNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWKL" gene 3682..4485 /gene="strA" CDS 3682..4485 /gene="strA" /codon_start=1 /transl_table=11 /product="Sm resistance protein A" /protein_id="AGT62528.1" /db_xref="GI:531997064" /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLL ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG" gene 4485..5321 /gene="strB" CDS 4485..5321 /gene="strB" /codon_start=1 /transl_table=11 /product="Sm resistance protein B" /protein_id="AGT62529.1" /db_xref="GI:531997065" /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT SY"ORIGIN 1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg 61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt 121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg 181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg 241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca 301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt 361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg 421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt 481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg 541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct 601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg 661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc 721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca 781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg 841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg 901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc 961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc 1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga 1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc 1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt 1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg 1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac 1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc 1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg 1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg 1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag 1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta 1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg 1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc 1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac 1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga 1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct 1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac 1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt 2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt 2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag 2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct 2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga 2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac 2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct 2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt 2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg 2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga 2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg 2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa 2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa 2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag 2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc 2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg 3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc 3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga 3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct 3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcatt 3601 catgtttgac agcttatcat ccataaggtt taatgcggta gtttatcaca gttaaattgc 3661 taacgcagtc aggcaccgtg tatgaatcga actaatattt tttttggtga atcgcattct 3721 gactggttgc ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg 3781 catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac 3841 cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag 3901 gaacaggagg gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg 3961 tctggagcgg atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac 4021 agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc 4081 gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt 4141 acgccgctgc acgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa 4201 gagcgcaccg atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac 4261 cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc 4321 tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa 4381 gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa 4441 cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg 4501 tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc 4561 tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta 4621 tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg 4681 gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc 4741 gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg 4801 aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga 4861 tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc 4921 aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac 4981 tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc 5041 tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct 5101 acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg 5161 acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt 5221 atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta 5281 tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct 5341 atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg 5401 attccgcccc ttcccgcaaa cgaccccaaa caagatcttg atcccctgcg ccatcagatc 5461 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc 5521 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc 5581 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag 5641 atagcccagt agctgacatt catcccaggt ggcacttttc ggggaaatgt gcgcgcccgc 5701 gttcctgctg gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct 5761 tttcgcccac ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc 5821 ccagggcgtc cagaacgggc ttcaggcgct cccgaaggt//
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