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-20℃
6个月
pENTR4
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pENTR4 |
质粒类型: | Gateway载体;入门载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | 无 |
克隆方法: | 限制性内切酶;多克隆位点 |
载体大小: | 2720 bp |
5' 测序引物及序列: | pENTR-F: CTACAAACTCTTCCTGTTAGTTAG |
3' 测序引物及序列: | pENTR-R: ATGGCTCATAACACCCCTTG |
载体标签: | 无 |
载体抗性: | 卡那霉素 |
真核筛选标记: | 无 |
备注: | pENTR4载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增; 构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。 |
病毒/非病毒: | 非病毒 |
LOCUS Exported 2720 bp ds-DNA circular SYN 13-AUG-2016DEFINITION synthetic circular DNAKEYWORDS pENTR4SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 2720) AUTHORS Biofeng Lab. TITLE Direct SubmissionFEATURES Location/Qualifiers source 1..2720 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 103..189 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" terminator 281..308 /note="rrnB T2 terminator" /note="transcription terminator T2 from the E. coli rrnB gene" protein_bind 358..457 /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL1" /note="recombination site for the Gateway(R) LR reaction" CDS 615..920 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="ccdB" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(949..1048) /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL2" /note="recombination site for the Gateway(R) LR reaction" CDS 1171..1980 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 2070..2658 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc 481 gactggatcc ggtaccgaat tcgcttacta aaagccagat aacagtatgc gtatttgcgc 541 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga 601 ggtgtgcttc tagaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc 661 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc 721 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 781 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta 841 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 901 tgatgttctg gggaatatag aattcgcggc cgcactcgag atatctagac ccagctttct 961 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca 1021 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa 1081 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 1141 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg 1201 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 1261 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg 1321 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 1381 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctggt 1441 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa 1501 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 1561 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 1621 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 1681 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca 1741 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 1801 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 1861 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 1921 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 1981 tcagaattgg ttaattggtt gtaacattat tcagattggg ccccgttcca ctgagcgtca 2041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 2101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 2161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 2221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 2281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 2341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 2401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 2461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 2521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 2581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 2641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 2701 tggccttttg ctcacatgtt
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