pRetroX-TetOne-luc

pRetroX-TetOne-luc

载体基本信息

出品公司:	Ybscience
载体名称:	pRetroX-TetOne-luc
质粒类型:	逆病毒载体；四环素调控载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	CMV IE
载体大小:	10144 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	--
克隆菌株:	DH5α
宿主细胞（系）:	常规细胞系（293、CV-1、CHO等）
备注:	逆病毒载体pRetroX-TetOne-luc是四环素诱导载体，作为pRetroX-TetOne载体的阳性参照。
稳定性:	稳表达
组成型/诱导型:	诱导型
病毒/非病毒:	逆转录病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pRetroX-TetOne-Luc          10144 bp ds-DNA    circular SYN 23-SEP-2013
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Catalog Nos. 634304, 634305, 634306

FEATURES             Location/Qualifiers

     LTR	     376..911
                     /label="CMV/MLV 5'LTR"
     misc_feature    974..1331
                     /label="Packaging Signal"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     polyA_signal    2298..2432
                     /label="SV40 polyA signal"
     CDS             complement(2613..4265)
                     /label="luciferase"
                     /note="firefly luciferase"                     
     promoter        complement(4275..4639)
                     /label="TRE3GS Promoter"
     promoter        4659..5169
                     /label="hPGK promoter"
     CDS             5188..5934
                     /label="Tet-On 3G"
     misc_feature    5952..6360
                     /label="WPRE"
                     /note="Partial WPRE. Contains alpha and gamma elements but 
                     no WHV protein X coding sequence"
     LTR	     6481..6728
                     /label="3'-LTR del in U3"
     promoter        8006..8110
                     /label="AmpR promoter"
     CDS             8111..8971
                     /label="AmpR"
     rep_origin      9142..9730
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagctagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat ataaagcatt
       61 tgacttgttc tatgcgccta ggagcttggc ccattgcata cgttgtatcc atatcataat
      121 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
      181 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      241 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      301 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      361 tgggtggagt atttacgcta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      421 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      481 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      541 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      601 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      661 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcat ctagagcgct
      721 tctgctcccc gagctctata aaagagccca caacccctca ctcggggcgc cagtcctccg
      781 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
      841 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
      901 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
      961 accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta
     1021 tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga
     1081 cccgtggtgg aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg
     1141 acttcggggg ccgtttttgt ggcccgacct gagtcctaaa atcccgatcg tttaggactc
     1201 tttggtgcac cccccttaga ggagggatat gtggttctgg taggagacga gaacctaaaa
     1261 cagttcccgc ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt
     1321 cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
     1381 gaaaatgagg gcccgggcta gcctgttacc actcccttaa gtttgacctt aggtcactgg
     1441 aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt
     1501 accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt
     1561 aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac
     1621 ccagaccagg tggggtacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg
     1681 gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc
     1741 ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct
     1801 tctctaggcg cccccatatg gccatatgag atcctatatg gggcaccccc gccccttgta
     1861 aacttccctg accctgacat gacaagagtt actaacagcc cctctctcca agctcactta
     1921 caggctctct acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa
     1981 caactggacc gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc
     2041 cgacaccaga ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc
     2101 acccccaccg ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag
     2161 gctgccgacc ccgggggtgg accatcctct agatcataat cagccatacc acatttgtag
     2221 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     2281 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaggcaata
     2341 gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt ggtttgtcca
     2401 aactcatcaa tgtatcttat catgtctgga tctcaaatcc ctcggaagct gcgcctgtct
     2461 taggttggag tgatacattt ttatcacttt tacccgtctt tggattaggc agtagctctg
     2521 acggccctcc tgtcttaggt tagtgaaaaa tgtcactctc ttacccgtca ttggctgtcc
     2581 agcttagctc gcaggggagg tggtctggat ccttacaatt tggactttcc gcccttcttg
     2641 gcctttatga ggatctctct gatttttctt gcgtcgagtt ttccggtaag acctttcggt
     2701 acttcgtcca caaacacaac tcctccgcgc aactttttcg cggttgttac ttgactggcg
     2761 acgtaatcca cgatctcttt ttccgtcatc gtctttccgt gctccaaaac aacaacggcg
     2821 gcgggaagtt caccggcgtc atcgtcggga agacctgcca cgcccgcgtc gaagatgttg
     2881 gggtgttgta acaatatcga ctccaattca gcgggggcca cctggtatcc tttgtattta
     2941 attaaagact tcaagcggtc aactatgaag aagtgttcgt cttcgtccca gtaagctatg
     3001 tctccagaat gtagccatcc atccttgtca atcaaggcgt tggtcgcttc cggattgttt
     3061 acataaccgg acataatcat aggtcctctg acacataatt cgcctctctg attaacgccc
     3121 agcgttttcc cggtatccag atccacaacc ttcgcttcaa aaaatggaac aactttaccg
     3181 accgcgcccg gtttatcatc cccctcgggt gtaatcagaa tagctgatgt agtctcagtg
     3241 agcccatatc cttgtcgtat ccctggaaga tggaagcgtt ttgcaaccgc ttccccgact
     3301 tctttcgaaa gaggtgcgcc cccagaagca atttcgtgta aattagataa atcgtatttg
     3361 tcaatcagag tgcttttggc gaagaatgaa aatagggttg gtactagcaa cgcactttga
     3421 attttgtaat cctgaaggga tcgtaaaaac agctcttctt caaatctata cattaagacg
     3481 actcgaaatc cacatatcaa atatccgagt gtagtaaaca ttccaaaacc gtgatggaat
     3541 ggaacaacac ttaaaatcgc agtatccgga atgatttgat tgccaaaaat aggatctctg
     3601 gcatgcgaga atctgacgca ggcagttcta tgcggaaggg ccacaccctt aggtaaccca
     3661 gtagatccag aggagttcat tatcagtgca attgttttgt cacgatcaaa ggactctggt
     3721 acaaaatcgt attcattaaa accgggaggt agatgagatg tgacgaacgt gtacatcgac
     3781 tgaaatccct ggtaatccgt tttagaatcc atgataataa ttttctggat tattggtaat
     3841 tttttttgca cgttcaaaat tttttgcaac ccctttttgg aaacaaacac tacggtaggc
     3901 tgcgaaatgt tcatactgtt gagcaattca cgttcattat aaatgtcgtt cgcgggcgca
     3961 actgcaactc cgataaataa cgcgcccaac accggcataa agaattgaag agagttttca
     4021 ctgcatacga cgattctgtg atttgtattc agcccatatc gtttcatagc ttctgccaac
     4081 cgaacggaca tttcgaagta ttccgcgtac gtgatgttca cctcgatatg tgcatctgta
     4141 aaagcaattg ttccaggaac cagggcgtat ctcttcatag ccttatgcag ttgctctcca
     4201 gcggttccat cctcaagagg atagaatggc gccgggcctt tctttatgtt tttggcgtct
     4261 tccatggtga attctttacg agggtaggaa gtggtacgga aagttggtat aagacaaaag
     4321 tgttgtggaa ttgaagttta ctcaaaaaat cagcactctt ttataggcgc cctggtttac
     4381 ataagcaaag cttatacgtt ctctatcact gatagggagt aaactggata tacgttctct
     4441 atcactgata gggagtaaac tgtagatacg ttctctatca ctgataggga gtaaactggt
     4501 catacgttct ctatcactga tagggagtaa actccttata cgttctctat cactgatagg
     4561 gagtaaagtc tgcatacgtt ctctatcact gatagggagt aaactcttca tacgttctct
     4621 atcactgata gggagtaaac tcgaggtgat aattccacgg ggttggggtt gcgccttttc
     4681 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     4741 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     4801 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     4861 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     4921 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     4981 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     5041 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     5101 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     5161 tctccccagg gggatcatcg aattaccatg tctagactgg acaagagcaa agtcataaac
     5221 tctgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
     5281 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     5341 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccactcctg ccccctggaa
     5401 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
     5461 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     5521 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     5581 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     5641 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaaa
     5701 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     5761 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
     5821 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     5881 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt
     5941 aagatcctgt taatcaacct ctggattaca aaatttgtga aagattgact gatattctta
     6001 actatgttgc tccttttacg ctgtgtggat atgctgcttt aatgcctctg tatcatgcta
     6061 ttgcttcccg tacggctttc gttttctcct ccttgtataa atcctggttg ctgtctcttt
     6121 atgaggagtt gtggcccgtt gtccgtcaac gtggcgtggt gtgctctgtg tttgctgacg
     6181 caacccccac tggctggggc attgccacca cctgtcaact cctttctggg actttcgctt
     6241 tccccctccc gatcgccacg gcagaactca tcgccgcctg ccttgcccgc tgctggacag
     6301 gggctaggtt gctgggcact gataattccg tggtgttgtc ggggaagctg acgtcgattc
     6361 ctgcggagat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     6421 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     6481 gaatgaaaga ccccacctgt aggtttggca agctagcttc tcgcttctgt tcgcgcgctt
     6541 ctgctcccag acatcaacaa aagagcccac aacccctcac tcggggcgcc agtcctccga
     6601 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     6661 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     6721 gtctttcacg cgtatgccgc atagttaagc caccgcggtg gcggtctgga ccacgccgga
     6781 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6841 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6901 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6961 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7021 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7081 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7141 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atggctccga
     7201 ccgaagccga cccgggcggc cccgccgacc ccgcacccgc ccccgaggcc caccgactct
     7261 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     7321 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     7381 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7441 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatggcg
     7501 cgtcgaatta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     7681 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     7741 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     7801 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7861 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgat gacgaaaggg
     7921 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8101 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8161 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8221 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8281 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8341 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8401 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8461 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8521 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8581 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8641 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8701 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8761 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8821 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8881 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8941 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     9301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     9541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9841 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9901 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9961 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10021 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    10081 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    10141 cgcc