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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
Tet-pLKO-neo
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
Tet-pLKO-neo
载体基本信息
| 出品人: | Ybscience |
|---|---|
| 载体名称: | Tet-pLKO-neo |
| 质粒类型: | 慢病毒载体；RNAi载体；四环素诱导载体；Tet-On载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶；多克隆位点 |
| 启动子: | -- |
| 载体大小: | 10959 bp |
| 5' 测序引物及序列: | 5’ GGCAGGGATATTCACCATTATCGTTTCAGA 3’ |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 感受态细胞 ，以维持质粒的稳定性。 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | 其它筛选标记 ： Tet-pLKO-puro |
| 产品目录号: | #21916 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 10959 bp ds-DNA circular SYN 10-AUG-2016
DEFINITION synthetic circular DNA
KEYWORDS Tet-pLKO-neo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10959)
AUTHORS Biofeng Lab.
FEATURES Location/Qualifiers
source 1..10959
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 106..210
/gene="bla"
/note="AmpR promoter"
CDS 211..1071
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1242..1830
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2118..2139
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2154..2184
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2192..2208
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2216..2232
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2253..2271
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2299..2525
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 2526..2706
/note="5' LTR"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2753..2878
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3371..3604
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
protein_bind 4170..4188
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4198..4216
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
misc_feature 6134..6251
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6300..6810
/note="PGK promoter"
/note="human phosphoglycerate kinase 1 promoter"
intron 6819..7391
/note="beta-globin intron"
/note="intron from rabbit beta-globin gene"
promoter 7446..7464
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 7475..8092
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/note="TetR"
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
misc_feature 8130..8703
/note="IRES"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 8710..9504
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
protein_bind complement(9533..9632)
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL2"
/note="recombination site for the Gateway(R) LR reaction"
LTR 9776..10009
/note="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 10081..10202
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 10242..10377
/note="SV40 ori"
/note="SV40 origin of replication"
promoter complement(10398..10416)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(10426..10442)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 10584..80
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
61 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
421 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
721 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
2101 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
2161 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
2221 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
2281 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
2341 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
2401 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
2461 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
2521 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
2581 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
2641 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
2701 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
2761 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
2821 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
2881 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
2941 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
3001 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
3061 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
3121 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
3181 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
3241 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
3301 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
3361 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
3421 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
3481 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
3541 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
3601 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
3661 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
3721 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
3781 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
3841 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
3901 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
3961 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
4021 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
4081 ctcgacggta tcgatcacga gactagcctc gagcggccgc aatatttgca tgtcgctatg
4141 tgttctggga aatcaccata aacgtgaaat ccctatcagt gatagagact tataagttcc
4201 ctatcagtga tagagacacc ggtgtgttgt aaatgagcac acaaaataca catgctaaaa
4261 tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata actttagtat
4321 caataattag aatttttatg ttcctttttg caaactttta ataaaaatga gcaaaataaa
4381 aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag ctactccacc
4441 acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt caaatgttct
4501 aactccacca gctgctttaa ctaaagcatt gtctttaaca actgacttca ttagtttaac
4561 atcttcaaat gttgcacctg attttgaaaa tcctgttgat gttttaacaa attctaatcc
4621 agcttcaaca gctatttcac aagctttcat gatttcttct tttgttaata aacaattttc
4681 cataatacat ttaacaacat gtgatccagc tgcttttttt acagctttca tgtcttctaa
4741 aactaattca taatttttgt cttttaatgc accaatattt aataccatat caatttctgt
4801 tgcaccatct ttaattgctt cagaaacttc gaatgctttt gtagctgttg tgcatgcacc
4861 tagaggaaaa cctacaacat ttgttattcc tacatttgtg ccttttaata attctttaca
4921 atagcttgtt caatatgaat taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc
4981 acataattgt ttaatttcag ctttcgtagc atcttgtttt aataatgtgt gatctatata
5041 tttgtttagt ttcatttttt ctcctatata ttcattttta attttaattc tttaataatt
5101 tcgtctactt taactttagc gttttgaaca gattcaccaa cacctataaa ataaattttt
5161 agtttaggtt cagttccact tgggcgaaca gcaaatcatg acttatcttc taaataaaat
5221 tttagtaagt cttgtcctgg catattatac attccatcga tgtagtcttc aacattaaca
5281 actttaagtc cagcaatttg agttaagggt gttgctctca atgatttcat taatggttca
5341 atttttaatt tcttttcttc tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca
5401 cccatttctt taaataaatc ttctaaatag tctactaatg ttttattttg ttttttataa
5461 aatcaagcag cctctgctat taatatagaa gcttgtattc catctttatc tctagctgag
5521 tcatcaatta catatccata actttcttca taagcaaaaa caaaatttaa tccgttatct
5581 tcttctttag caatttctct acccattcat ttaaatccag ttaaagtttt tacaatatta
5641 actccatatt tttcatgagc gattctatca cccaaatcac ttgttacaaa acttgaatat
5701 agagccggat tttttggaat gctatttaag cgttttagat ttgataattt tcaatcaatt
5761 aaaattggtc ctgtttgatt tccatctaat cttacaaaat gaccatcatg ttttattgcc
5821 attccaaatc tgtcagcatc tgggtcattc ataataataa tatctgcatc atgtttaata
5881 ccatattcaa gcggtatttt tcatgcagga tcaaattctg gatttggatt tacaacattt
5941 ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa taacgttata tcctgattca
6001 cgtaatattt ttggggtaaa tttagttcct gttccattaa ctgcgctaaa aataattttt
6061 aaatcttttt tagcttcttg ctcttttttg tagaattctc gacctcgaga caaatggcag
6121 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
6181 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
6241 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg
6301 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
6361 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
6421 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
6481 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
6541 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
6601 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
6661 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
6721 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
6781 gttgaccgaa tcaccgacct ctctccccag ggggatctgt gagtttgggg acccttgatt
6841 gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg
6901 tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt
6961 tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg
7021 taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt
7081 atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat
7141 tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa
7201 tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca
7261 tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg tttgagatga
7321 ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct
7381 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
7441 aattgtaata cgactcacta tagggcgaat tgatatgtct agattagata aaagtaaagt
7501 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
7561 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
7621 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
7681 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
7741 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
7801 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
7861 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
7921 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
7981 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
8041 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgcgta
8101 cagcggctcc cgggagttct agggatctgc ccctctccct cccccccccc taacgttact
8161 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
8221 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
8281 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
8341 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
8401 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
8461 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
8521 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
8581 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
8641 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
8701 taaggatcca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
8761 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
8821 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
8881 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
8941 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
9001 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
9061 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
9121 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
9181 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
9241 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
9301 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
9361 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
9421 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
9481 cgccttcttg acgagttctt ctgaggtacc ttttttaagg gtgggcgcgc cgacccagct
9541 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
9601 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccg cccgccccac
9661 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca
9721 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
9781 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
9841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
9901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
9961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
10021 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
10081 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
10141 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
10201 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
10261 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
10321 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
10381 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
10441 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
10501 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
10561 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
10621 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
10681 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
10741 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
10801 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
10861 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
10921 ctcggtctat tcttttgatt tataagggat tttgccgat
//
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文献和实验相关实验
-selection cassette (RpsL -neo) The Red/ET recombination system (pSC101BADγβαA[tet]), and the RpsL -neo counter-selection cassette (CS) were purchased from Genebridges, Dresden, Germany. BAC clones BACs containing
Amp Tet-pLKO-Puro 哺乳细胞质粒 四环素调控系统 pTRE2pur 哺乳细胞质粒 Amp Tet-on，Tet-off psiCHECK2 哺乳细胞质粒 双荧光分析质粒
动物细胞表达外源基因时，大多数情况下不需要诱导，但当表达产物对细胞有毒性时应采取诱导，这样可避免表达产物产生早期就对细胞产生影响。哺乳动物细胞中用到的诱导型载体主要与启动子有关如热休克蛋白启动子可在高温下被诱导，还有重金属、糖皮质激素诱导的启动子。但这些系统存在一些共同的缺陷，如诱导表达特异性差;当系统处于关闭状态时表达有泄漏诱导剂本身有毒性，常对细胞造成损伤等。 为此，Gossen等构建了受四环素负调节的Tet-on基因表达系统，该系统由调节质粒和反应质粒组成。调节质粒中具有编码转录
技术资料暂无技术资料 索取技术资料
Tet-pLKO-neo
¥800 - 1200
