pFLAG-CMV-5a

pFLAG-CMV-5a

载体基本信息

出品公司:	Ybscience
载体名称:	pFLAG-CMV-5a
质粒类型:	哺乳动物表达载体
高拷贝/低拷贝:	低拷贝
克隆方法:	多克隆位点；限制性内切酶
启动子:	CMV
载体大小:	4.7 kb
5' 测序引物及序列:	CMV Forward：CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	--
载体标签:	FLAG tag (C-末端）
载体抗性:	氨苄青霉素
筛选标记:	无
克隆菌株:	--
宿主细胞（系）:	常用细胞系，如293、Hela等
备注:	CMV启动子驱动目的蛋白高水平表达； FLAG标签含8个氨基酸残基（DYKDDDDK） 一般位于融合蛋白的外表面，具有高度免疫灵敏性，便于检测和纯化目的蛋白；
稳定性:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

Product Description
Versions of the pFLAG-CMV-5 expression vector for the three translational reading frames preceding the FLAG coding sequence are provided.

pFLAG-CMV-5a expression vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of C-terminal FLAG fusion proteins in mammalian cells.

The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-fusion constructs.
The multiple cloning region of the pFLAG-CMV-5a expression vector is not preceded by a translational initiation signal. A translational start sequence3 must be included with the insert DNA.
pFLAG-CMV-5a expression vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.


p-FLAG-CMV-5a, p-FLAG-CMV-5b, and p-FLAG-CMV-5c Expression Vectors used for the expression of C-terminal FLAG® tagged proteins in mammalian cells. pFLAG-CMV-5 a,b,c Expression Vectors are used as shuttle vectors for E. coli and mammalian cells. These vectors facilitate the intracellular expression of C-terminal FLAG-tagged fusion proteins. Transient expression in mammalian cells is driven by the CMV promoter-regulatory region. The SV40 origin of replication results in most efficient replication in mammalian cells expressing the SV40 large T antigen. The a, b, and c vector series makes all three reading frames available for every restriction site in the MCS