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-20℃
6个月
pCMV-SPORT1
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pCMV-SPORT1 |
质粒类型: | 哺乳动物表达载体;cDNA克隆载体;I.M.A.G.E.载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 4380 bp |
5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 哺乳动物细胞 |
备注: | 用于cDNA文库的构建 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
LOCUS Exported 4380 bp ds-DNA circular SYN 28-FEB-2013DEFINITION Vector for cloning and transient mammalian cell expression of cDNAs.ACCESSION .VERSION .KEYWORDS pCMV SPORTSOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 4380) AUTHORS I.M.A.G.E. ConsortiumFEATURES Location/Qualifiers source 1..4380 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" polyA_signal 125..259 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" promoter 646..664 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 682..779 /note="MCS" /note="multiple cloning site" promoter complement(787..805) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" promoter complement(921..1124) /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" enhancer 1125..1428 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" protein_bind 1458..1474 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1482..1513) /note="lac UV5 promoter" /note="E. coli lac promoter with an ""up"" mutation" rep_origin complement(1975..2563) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2641..2674 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(2776..3636) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin complement(3924..4379) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta 361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct 421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact 481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta 541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca 601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcttataata cgactcacta 661 tagggagaga gctatgacgt cgcatgcacg cgtaagcttg ggcccctcga gggatcctct 721 agagcggccg ccgactagtg agctcgtcga cccgggaatt ccggaccggt acctgcaggc 781 gtaccttcta tagtgtcacc taaatagctt tttgcaaaag cctaggctag agtccggagg 841 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg 901 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg 961 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg 1021 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca 1081 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg 1141 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa 1201 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac 1261 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg 1321 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg 1381 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgac ctgcacgatg 1441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggaagc 1501 tataaagtgt aaagcctggg gtgcctaatg agtgaaaggg cctcgtatac gcctattttt 1561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 1621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 1681 gagacaataa ccctgataaa tgcttcaata atattgaaaa acgcgcgaat tgcaagctct 1741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 1801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 1921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 1981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 2101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2161 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 2401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 2461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 2521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 2581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc 2641 ataacttcgt atagcataca ttatacgaag ttatggcatg agattatcaa aaaggatctt 2701 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 2761 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 2821 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 2881 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2941 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 3001 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 3061 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 3121 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 3181 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3241 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3301 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3361 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 3421 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3481 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3541 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3601 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 3661 aagcatttat cagggttatt gtctcatgcc aggggtgggc acacatattt gataccagcg 3721 atccctacac agcacataat tcaatgcgac ttccctctat cgcacatctt agacctttat 3781 tctccctcca gcacacatcg aagctgccga gcaagccgtt ctcaccagtc caagacctgg 3841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 3901 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt 3961 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 4021 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 4081 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 4141 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 4201 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 4261 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 4321 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
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