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- GMS80175.2
- 2025年08月19日
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extract more than 50 minipreps at once. Procedures of RNA extraction published so far follow
Protocol: Fast yeast transformation Add 50 µl carrier DNA to a 1.5 ml tube. scrap cells from plate and add to the carrier DNA. Add in the following order: 1-2 µl DNA-plasmid (up to 30-50 µl) 240 µ
“The determination of the three-dimensional structures of protein molecules showed for the first time in detail the construction of the molecular ‘machines’ of the life cycle. If we want to learn how these ‘machines’ work
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