手机验证
-20℃
6个月
pCDFDuet-1
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
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质粒类型: | 大肠杆菌蛋白双表达载体,有两个多克隆位点 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 3781 bp |
5' 测序引物: | T7 |
5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; |
3' 测序引物: | T7t |
3' 测序引物序列: | T7t: 5'-TGCTAGTTATTGCTCAGCGG-3' |
载体标签: | N-His, C-S |
载体抗性: | streptomycin |
备注: | pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA). |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pCDFDue-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.
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