pCDFDuet-1

pCDFDuet-1

pCDFDuet1载体基本信息

出品公司:	Ybscience
质粒类型:	大肠杆菌蛋白双表达载体，有两个多克隆位点
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3781 bp
5' 测序引物:	T7
5' 测序引物序列:	T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物:	T7t
3' 测序引物序列:	T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签:	N-His, C-S
载体抗性:	streptomycin
备注:	pCDFDuet-1 is designed for the coexpression of two target genes. pCDFDuet-1 carries the CloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance gene (aadA).
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pCDFDuet1载体质粒图谱和多克隆位点信息

pCDFDuet1载体简介

pCDFDue-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the CloDF13-derived CDF replicon, lacI gene and streptomycin/spectinomycin resistance gene (SmR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pRSFDuet™-1 (Cat. No. 71341-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.

pCDFDuet1载体序列

LOCUS       pCDFDuet    3781 bp     DNA Circular    SYN
DEFINITION  pCDFDuet
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="pCDFDuet"
                     /mol_type="other DNA"
     misc_feature    2..29
                     /label="lacO"
     promoter        214..232
                     /label="T7_promoter"
     misc_feature    232..259
                     /label="lacO"
     misc_feature    complement(448..466)
                     /label="T7_Terminal_primer"
     terminator      467..502
                     /label="T7_terminator"
     gene            complement(680..1471)
                     /label="spect (variant)"
                     /gene="spect (variant)"
     CDS             complement(680..1471)
                     /label="ORF frame 1"                  
     CDS             complement(680..1471)
                     /label="ORF frame 1"                  
     promoter        complement(1513..1541)
                     /label="AmpR_promoter"                  
     misc_feature    complement(2559..3650)
                     /label="lacI"                   
     CDS             complement(2559..3518)
                     /label="ORF frame 3"                  
     CDS             complement(2559..3518)
                     /label="ORF frame 3"                  
     CDS             complement(3391..158)
                     /label="ORF frame 3"                  
     CDS             complement(3391..158)
                     /label="ORF frame 3"                 
     promoter        3765..2
                     /label="T7_promoter"                  
ORIGIN
    1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
   61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
  121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
  181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
  241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
  301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
  361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
  421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
  481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
  541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
  601 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc
  661 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
  721 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
  781 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
  841 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
  901 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
  961 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
 1021 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
 1081 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
 1141 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
 1201 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
 1261 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
 1321 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
 1381 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
 1441 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca
 1501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
 1561 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
 1621 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
 1681 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
 1741 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
 1801 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct
 1861 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac
 1921 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
 1981 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
 2041 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc
 2101 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
 2161 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
 2221 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag
 2281 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat
 2341 cctttgatct tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta
 2401 gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg
 2461 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
 2521 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
 2581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
 2641 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
 2701 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
 2761 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
 2821 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
 2881 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
 2941 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
 3001 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
 3061 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
 3121 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
 3181 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
 3241 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
 3301 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
 3361 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
 3421 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
 3481 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
 3541 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
 3601 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
 3661 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
 3721 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat
 3781 a