手机验证
-20℃
长期
2×PCR Master Mix
896
北京百奥莱博科技有限公司
400次
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博供应的2×PCR Master Mix(含Taq酶,buffer,dNTP)北京厂家用于科研目的生化试验项目,本产品质量好,且极具价格优势,深为用户称道,了解更多2×PCR Master Mix(含Taq酶,buffer,dNTP)等核酸扩增(PCR)产品请联系我司咨询订购。
名称:2×PCR Master Mix(含Taq酶,buffer,dNTP)北京厂家
编号:YT378
产地:国产|进口
英文名:2×PCR Master Mix
品牌:百奥莱博
2X PCR Master Mix中含有2X Taq DNA Polymerase,2X PCR Buffer,2XdNTP,只需加入适量引物、模板和水即可进行PCR扩增。大大简化了PCR操作,使操作更加快捷,也减少了PCR操作过程中可能导致的污染,使PCR的重复性更好。
利用2X PCR Master Mix可以进行各种常规的PCR扩增,特别适合用于定性和半定量的PCR扩增,以及2kb以下DNA*(代"片")段的T载体克隆。
2X PCR Master Mix可以扩增出长达8kb的片段,但通常更适合用于扩增2kb以下的DNA*(代"片")段。
本产品稳定性高,经测试,反复冻融15次后对PCR的扩增效果无显著影响。
本试剂盒用于50微升的PCR反应体系,足够用于160个反应;用于20微升的PCR反应体系,足够用于400个反应。
注意事项:
1)由于PCR反应非常灵敏可以扩增目的基因序列超过1000万倍,在使用Taq酶时请注意避免微量待扩增DNA的污染,并尽量考虑设置不加模板的空白对照以确认是否有待扩增DNA的污染。
2)Taq DNA polymerase在PCR过程中每循环的出错几率约为2.2×10-5,对于大于1kb的DNA*(代"片")段的克隆推荐使用出错几率更低的DNA聚合酶,例如Pfu DNA polymerase、YT Taq DNA polymerase等。对于普通的PCR或RT-PCR定性检测或定量检测,Taq DNA polymerase是最佳选择。
3)尽管本产品经过15次反复冻融后仍具有和冻融前几乎相同的PCR扩增效果,但仍宜适当避免反复冻融本产品,多次反复冻融可能使产品性能下降。
4)使用本产品前,一定要完全融化,并上下颠倒轻轻混匀后才能使用,尽量避免起泡。
储存条件:-20℃。
除2×PCR Master Mix(含Taq酶,buffer,dNTP)北京厂家外,我公司正在打折促销以下产品:
9035-81-8 Trypsin inhibitor 胰蛋白酶抑制剂
PY02-397 APT琼脂 250克
ARB12139 大鼠生长激素(GH)elisa检测操作说明书 Rat growth hormone,gh ELISA KIT
2,2’-联喹啉-4,4’-二羧酸钠 Trans-zeatin Riboside 979-88-4
ARB10302 人巨噬细胞刺激蛋白(MSP)定量分析 Human macrophage stimulating protein,msp ELISA KIT
ARB12891 小鼠甲状腺球蛋白(TG)Elisa分析 Mouse thyroglobulin,tg ELISA KIT
ARB13185 小鼠胰岛素样生长因子1(IGF-1)血清中含量检测 Mouse insulin-like growth factor 1,igf-1 ELISA KIT
ARB12000 大鼠P选择素(P-Selectin/CD62P)尿液中含量检测 Rat P-Selectin ELISA KIT
BTN80204 柱式RNA纯化试剂盒 Column RNACLEAN
BL1300 pUC18
ARB11419 人载脂蛋白A1(Apo-A1)含量检测 Human apoprotein a1,apo-a1 ELISA KIT
ARB12974 小鼠多巴胺(DA)酶标法分析 Mouse dopamine,da ELISA KIT
ARB11184 人视黄酸诱导基因/RIG-1样受体(RLR)含量分析 Human retinoic acid inducible gene/rig-like receptor,rlr-9 ELISA KIT
ARB12287 大鼠抗子宫内膜抗体(EMAb)Elisa分析 Rat endometrium antibody,emab ELISA KIT
ARB10670 人血管紧张素Ⅱ受体2抗体(AT2R-Ab)elisa检测 Human angiotensin Ⅱ receptor 2 antibody,at2r-ab ELISA KIT
PY01-166 溴甲酚紫 ind 10克
ARB12928 小鼠皮质酮(CS)elisa检测操作说明书 Mouse corticosterone,cs ELISA KIT
1397-89-3 可溶性两性霉素B Amphotericin B solubilized
ARB10156 人S100蛋白(S-100)elisa测定使用说明书 Human soluble protein-100,s-100 ELISA KIT
2×PCR Master Mix(含Taq酶,buffer,dNTP)北京厂家关键词:2×PCR Master Mix(含Taq酶,buffer,dNTP),YT378
·兔抗VCP抗体
编号:YT767
英文名称:VCP Rabbit Polyclonal Antibody
规格:50μl
本品为来源于兔的抗VCP的多克隆抗体。
宿主:兔
克隆性:多克隆抗体
用途:WB,ICC, IHC,IF
交叉反应性:人,小鼠
分子量:89KDa
推荐稀释比例:WB(1:1000),IF(1:100),IHC(1:100),ICC(1:100)
About this Antibody | |
Name | VCP Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to VCP. |
Gene ID | 7415 |
SwissProt | P55072 |
Synonyms | VCP; IBMPFD; MGC131997; MGC148092; MGC8560; TERA; p97 |
Category | Ubiquitin and Ubiquitin-like proteins |
Background | Valosin-containing protein (VCP) is a highly conserved, that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. VCP is involved in the formation of the transitional endoplasmic reticulum (tER). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome. |
About this Antibody | |
Name | β-Catenin Rabbit Polyclonal Antibody |
Category | Polyclonal antibody(pAb); Primary antibody |
Isotype | IgG |
Purification | Peptide affinity purified |
About the Immunogen | |
Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to β-catenin. |
Gene ID | 1499 |
SwissProt | P35222 |
Synonyms | CTNNB; DKFZp686D02253; FLJ25606; FLJ37923; β-catenin; Beta-Catenin |
Category | Developmental Biology |
Background | β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. |
我公司生产供应销售的核酸扩增(PCR)产品正全系列现货促销,满分期待您的垂询选购2×PCR Master Mix(含Taq酶,buffer,dNTP)北京厂家。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。