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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Tetranectin purified from human citrate plasma (3) and coupled to PPD. Boosted before fusion with recombinant tetranectin produced in E. coli.
- 亚型:
Mouse IgG1/k
- 形态:
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
H
- 宿主:
Mouse
- 应用范围:
E, WB
- 浓度:
见详细说明信息
- 靶点:
Tetranectin (10E3)
- 抗体英文名:
Tetranectin (10E3) Antibody
- 抗体名:
Tetranectin (10E3)抗体
- 规格:
0.2 mg
Tetranectin (10E3) Antibody产品详细介绍:
| 产品名称: | Tetranectin (10E3) Antibody |
| 说明书: | http://www.abbiotec.com/antibodies/tetranectin-10e3-antibody【参考图片】 |
| 货号: | 250351 |
| 规格: | 0.2 mg |
| 产品描述: | Tetranectin (TN) is a serum and tissue protein, a C-type lectin, which binds to Ca++. It is a homotrimer of monomers each with a mass of 20 kDa, plasma or serum concentrations of TN are found to be approximately 10 mg/l. In vitro, TN can bind to kringle 4 of plasminogen and enhance the activation of plaminogen to plasmin, catalyzed by tissue plasminogen activator in the presence of poly-D-lysine. TN is best known as a prognostic marker in ovarian cancer. This antibody is specific for amino acids 17-181 of human tetranectin monomer. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1/k |
| 免疫原: | Tetranectin purified from human citrate plasma (3) and coupled to PPD. Boosted before fusion with recombinant tetranectin produced in E. coli. |
| 适用物种: | H |
| 应用范围: | E, WB |
| 应用说明: | Product Cat. No. 250351 reacts strongly with tetranectin in sandwich ELISA in combination with a polyclonal antibody against tetranectin. Product Cat. No. 250351 reacts strongly in western blot with TN monomer and slightly with TN trimer. Product Cat. No. 250351 shows no staining for TN in fresh frozen tissues of ovarian cancer, and paraffin-embeded, microwave treated tissues. E: 1:8,000; WB: 1:1,000 |
| 别名: | TN; C-type lectin domain family 3 member B; Plasminogen kringle 4-binding protein |
| 形态: | Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件: | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验Yeast IF without Dehydration--酵母免疫荧光(无甲醇脱水方法)
Note :this protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37% formaldehyde (best grade) and incubate
Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37 formaldehyde (best grade) and incubate on the roller at same temp. for 10 min. Spin down cells 2Kx3min. and resuspend in 5mls 40mMKPO4 pH6.5/500uM
Yeast IF without Dehydration David Amberg Note this protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add
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