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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme.
- 亚型:
Mouse IgG1/k
- 形态:
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
H, B, Ml, Other
- 宿主:
Mouse
- 应用范围:
E, WB
- 浓度:
见详细说明信息
- 靶点:
Acetylcholinesterase (4E11)
- 抗体英文名:
Acetylcholinesterase (4E11) Antibody
- 抗体名:
Acetylcholinesterase (4E11)抗体
- 规格:
0.2 mg
Acetylcholinesterase (4E11) Antibody产品详细介绍:
| 产品名称: | Acetylcholinesterase (4E11) Antibody |
| 说明书: | http://www.abbiotec.com/antibodies/acetylcholinesterase-4e11-antibody【参考图片】 |
| 货号: | 250379 |
| 规格: | 0.2 mg |
| 产品描述: | Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa. Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects. This antibody is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE. There is a weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1/k |
| 免疫原: | C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme. |
| 适用物种: | H, B, Ml, Other |
| 应用范围: | E, WB |
| 应用说明: | Product Cat. No. 250379 can be used in ELISA on amniotic fluid for the diagnosis of neural tube defects. Product Cat. No. 250379 is used as capture antibody (immobilized with an anti-mouse IgG) in enzyme antigen immunoassay (EAIA), in which the AChE is captured and used directly as substrate for acetylthiocholiniodide (Ellmann’s reaction). Product Cat. No. 250379 reacts in western blot with native and denatured human and bovine detergent-soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE. E: 1:250; WB: 1:75 |
| 别名: | AChE |
| 形态: | Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件: | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验Preparation and Assay of Acetylcholinesterase Antibody
structures mimicking external antigen results from the fact that the conformation of some of the anti-idiotypic antibodies may be complementary to the three-dimensional structure of the binding site of the first antibody, and therefore, represents
Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo
in the intraembryonic aorta-gonad-mesonephros region at embryonic day (E) 10.5. After E11, these HSCs are thought to seed the liver and then the bone marrow just before birth. So far, many questions concerning the origin, properties, and functionality of these HSCs
Wholemount in situ hybridisation
. Each batch should ideally be tested. As a rough guide, use 5 min for E7.5, 7 min for E8.5, 9 min for E9.5, 11 min for E10.5, 12-14 min for E11.5. 8 Wash twice with PBTX for 5 minutes each. Be careful - the embryos are fragile
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