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文献和实验Dual- and Triple-Color Fluorospot
can also be adjusted to single staining by using reagents for only one cytokine. 1. Dual-Color Fluorospot 1) Preparation of the plates and coating for double staining (sterile conditions) a. Pre-wet a sterile 96-well PVDF plate with 20 μl
Additional reagents and equipment for PCR (unit 15.1 ), restriction endonuclease digestion (unit 3.1 ), agarose gel electrophoresis (unit 2.5 ), purification of DNA fragments (unit 2.6 ), lithium acetate transformation of yeast
when temperature of the cover becomes lower than temperature of the liquid in the plate. It is convenient to use an air fan to accelerate thawing. #6. 12.2ml per plate, if PCR volume is 30µl/well (maximum for old ABT-384
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