Recombinant Mouse PD-L1/B7-H1

Recombinant Mouse PD-L1/B7-H1/CD274  (CJ89)

 

产品说明(Description)

Recombinant Mouse Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a Fc tag at the C-terminus. 

Accession #: Q9EP73

Known as: Programmed cell death 1 ligand 1Cd274;  programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;programmed death ligand 1;B7 homolog 1;B7-H1;CD274; 

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

 

质量控制(Quality Control) 

Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:	Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:

 

Immobilized Mouse PD-L1-Fc(Cat#CJ89) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb-FC*.*: Biotinylated by NHS-biotin prior to testing.The ED50 of Anti-Human PDL1 mAb-FC* is 0.008 ug/ml.*: Biotinylated by NHS-biotin prior to testing.	000000	Immobilized Mouse PD-L1-Fc(Cat#CJ89) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb-FC*.*: Biotinylated by NHS-biotin prior to testing.The ED50 of Anti-Human PDL1 mAb-FC* is 0.008 ug/ml.*: Biotinylated by NHS-biotin prior to testing.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Mouse Programmed cell death 1 ligand 1(Cd274,PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Poor outcome with anti-programmed death-ligand 1 (PD-L1) antibody due to poor pharmacokinetic properties in PD-1/PD-L1 blockade-sensitive mouse models[J]. Taiki Kurino. et al. Journal for ImmunoTherapy of Cancer. 2020.

Effects of MAPK and PI3K Pathways on PD-L1 Expression in Melanoma[J]. Atefi M S. et al. Clinical Cancer Research. 2014.

 

 

FOR RESEARCH USE ONLY