Recombinant Human TIM-3 (C-6His) (C356)
产品说明(Description)
Recombinant Human T-Cell Membrane Protein 3 is produced by our Mammalian expression system and the target gene encoding Ser22-Arg200 is expressed with a 6His tag at the C-terminus.
Accession #: AAL65157.1
Known as: Hepatitis A Virus Cellular Receptor 2; HAVcr-2; T-Cell Immunoglobulin and Mucin Domain-Containing Protein 3; TIMD-3; T-Cell Membrane Protein 3; TIM-3; HAVCR2; TIM3; TIMD3
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Bioactivity-ELISA: |
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Bioactivity-BLI: |
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Immobilized Anti-Human TIM-3 mAb(Cat#NC056) at 2μg/ml (100 μl/well) can bind Human TIM-3-His (Cat#C356). The ED50 of Recombinant Human TIM-3-His (Cat#C356) is 8.51ng/ml. |
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Loaded Anti-Human TIM-3 mAb-Fc(Cat#NC056) on Protein A Biosensor, can bind Human TIM-3-His(Cat#C356) with an affinity constant of 0.12 nM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
T-Cell Membrane Protein 3 (TIM3) is a single-pass type I membrane protein that belongs to the TIM family of immunoglobulin superfamily. TIM3 includes a signal sequence (aa 1-21), an extracellular region (aa 22-202) with one Ig-like V-type domain, a transmembrane segment (aa 203-223), and a cytoplasmic domain (aa 224 - 301). TIM3 regulates macrophage activation, inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. It may be also involved in T-cell homing and as a receptor for LGALS9.
电泳(SDS-PAGE)
参考文献(Reference)
Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody[J]. Dong Zhang. et al. OncoImmunology. 2020.
FOR RESEARCH USE ONLY