Biotinylated Human CD47 (C-Fc-

Biotinylated Human CD47 (C-Fc-Avi) (CU01)

产品说明(Description)

Biotinylated Recombinant Human Leukocyte Surface Antigen CD47 is produced by our Mammalianexpression system and the target gene encoding Gln19-Pro139 is expressed with a Fc, Avi tag at the C-terminus.

Accession #: Q08722

Known as: Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47; MER6     

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.

 

质量控制(Quality Control)

Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:	Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Biotin:Protein Ratio        The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Elisa:

 

Immobilized Human CD47-Fc-Avi (Cat#CU01) at 10μg/ml (100 μl/well) can bind Human SIRPA-His(Cat#C385). The ED50 of Recombinant Human SIRPA-His(Cat#C385) is 17.3 ng/ml.	000000	Immobilized Human CD47-Fc-Avi (Cat#CU01) at 10μg/ml (100 μl/well) can bind Human SIRPA-His(Cat#C385). The ED50 of Recombinant Human SIRPA-His(Cat#C385) is 17.3 ng/ml.
HPLC:
The purity of Biotinylated Human CD47-Fc-Avi (Cat#CU01) is greater than 95% as determined by SEC-HPLC.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

CD47(Integrin-Associated Protein,IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY