Recombinant Human CD47/IAP/OA3 (C321)
产品说明(Description)
Recombinant Human Leukocyte Surface Antigen CD47 is produced by our Mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a 6His tag at the C-terminus.
Accession #: Q08722
Known as: Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0.
质量控制(Quality Control)
Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin: |
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
Bioactivity-Elisa:
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Immobilized Human SIRPA-Fc (Cat#CP67) at 10μg/ml (100 μl/well) can bind Human CD47-His (Cat#C321). The ED50 of Human CD47-His(Cat#C321) is 13-89 ng/ml. |
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Immobilized Human CD47-His(Cat#C321) at 10μg/ml (100 μl/well) can bind Anti-Human CD47 mAb-IgG4Fc(Cat#NC011). The ED50 of Anti-Human CD47 mAb-IgG4Fc(Cat#NC011) is 19.9 ng/ml. |
HPLC: |
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Bioactivity-BLI: |
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The purity of Human CD47-His (Cat#C321) is greater than 95% as determined by SEC-HPLC. |
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Loaded Anti-Human CD47 mAb-Fc(Cat#NC011) on Protein A Biosensor, can bind Human CD47-His(Cat#C321) with an affinity constant of 0.25 nM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
CD47(Integrin-Associated Protein,IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
电泳(SDS-PAGE)
参考文献(Reference)
Novel Pharmaceutical Strategy for Selective Abrogation of TSP1-Induced Vascular Dysfunction by Decoy Recombinant CD47 Soluble Receptor in Prophylaxis and Treatment Models[J]. Molly Yao. et al. Biomedicines. 2021.
A single-valent long-acting human CD47 antagonist enhances antibody directed phagocytic activities[J]. Fenglan Wu. et al. CANCER IMMUNOLOGY IMMUNOTHERAPY. 2020.
CD47 ANTIGEN BINDING UNIT AND USES THEREOF[J]. Qiu Yangsheng. et al. 2020.
FOR RESEARCH USE ONLY