Recombinant Human Quinolinate Phosphoribosyltransferase [Decarboxylating] is produced by our E.coli expression system and the target gene encoding Met1-His297 is expressed with a 6His tag at the N-terminus. Accession #: AAH05060.1 Known as: Nicotinate-Nucleotide Pyrophosphorylase [Carboxylating]; Quinolinate Phosphoribosyltransferase [Decarboxylating]; QAPRTase; QPRTase; QPRT
制剂(Formulation) Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.
质量控制(Quality Control)
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping) The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
保存(Storage) Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
背景(Background) Nicotinate-Nucleotide Pyrophosphorylase (QPRT) belongs to the nadC/modD family. QPRT plays an improtant role in catabolism of quinolinate which acts as a potent endogenous exitotoxin to neurons. In addition, QPRT serves as an an intermediate in the Tryptophan-Nicotinamide Adenine Dinucleotide pathway. QPRT participates in some pathways including Cofactor biosynthesis, NAD(+) biosynthesis and the Nicotinate D-Ribonucleotide from Quinolinate. In addition, QPRT is involved in the catabolism of Quinolinic Acid (QA). The activity toward QA is slightly repressed by phosphoribosylpyrophosphate (PRPP) in both a competitive and a non-competitive manner.