Recombinant Human Proteinase 3/Myeloblastin/PRTN3 (C628)
产品说明(Description)
Recombinant Human Proteinase 3 is produced by our Mammalian expression system and the target gene encoding Ala26-Arg249 is expressed with a 6His tag at the C-terminus.
Accession #: P24158
Known as: Myeloblastin; AGP7; C-ANCA Antigen; Leukocyte Proteinase 3; PR-3; PR3; Neutrophil Proteinase 4; NP-4; P29; Wegener Autoantigen; PRTN3; MBN
制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 10mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
Proteinase-3 is a neutral serine proteinase that belongs to the peptidase S1 family and Elastase subfamily. It contains one peptidase S1 domain and it is expressed mainly in neutrophil granulocytes. The primary function of Proteinase-3 is thought to be degradation of extracellular proteins at sites of inflammation, but excessive or prolonged proteolytic activity may cause harmful effects in the body. It is the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis.
电泳(SDS-PAGE)
参考文献(References)
Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation[J]. Yansen Xiao. et al. Cancer Cell. 2021.
Gasdermin D plays a vital role in the generation of neutrophil extracellular traps[J]. Gabriel Sollberger. et al. Science Immunology. 2018.
Activation of C3a receptor is required in cigarette smoke-mediated emphysema[J]. X Yuan. et al. Mucosal immunology. 2015.
FOR RESEARCH USE ONLY