Recombinant Human Pro-Neuregulin-1/NRG1-β1/HRG1-β1 (Met1-Lys246) (CH84)
产品说明(Description)
Recombinant Human Neuregulin-1 beta is produced by our E.coli expression system and the target gene encoding Met1-Lys246 is expressed with a 6His tag at the N-terminus.
Accession #: AAA58639.1
Known as: Pro-neuregulin-1;Neuregulin-1 beta 1;NRG1-beta 1;HRG1-beta 1 ECD;EGF;NRG1; GGF; HGL; HRGA; NDF; SMDF;
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 4mM HCl.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: Immobilized Human NRG1Beta-His (Cat#CH84) at 10μg/ml (100 μl/well) can bind Human HER3-Fc (Cat#CX93).
The ED50 of Human Glypican-3-His(Cat#CY15) is 5.18 ug/ml.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 4mM HCl
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
neuregulin-1 (heregulin-1,NRG1) is a member of neuregulin family, which is comprised of four genes that encode a large number of secreted or membrane-bound isoforms. All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. NRG1 isoforms can be classified into type I, type II and type III isoforms. NRG1 directs ligand for ERBB3 and ERBB4 tyrosine kinase receptors, concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. NRG proteins show distinct spatial and temporal expression patterns and play important roles during development of both the nervous system and the heart.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY