Recombinant Human Neuroligin 1/NLGN1(46-693) (C493)
产品说明(Description)
Recombinant Human Neuroligin 1 is produced by our Mammalian expression system and the target gene encoding Gln46-Leu676 is expressed with a 6His tag at the C-terminus.
Accession #: Q8N2Q7-2
Known as: Neuroligin-1; NLGN1; KIAA1070
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Neuroligin-1 is a single-pass type I transmembrane protein which belongs to the type-B Carboxylesterase/Lipase family. Neuroligins are cell-adhesion molecules located at the postsynaptic side of the synapse. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses. Neurexins and Neuroligins are cell adhesion molecules present in excitatory and inhibitory synapses, and they are required for correct neuron network function. These proteins are found at the presynaptic and postsynaptic membranes. Neuroligin-1 is a neuronal cell surface protein which is thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. It seems to play role in formation or maintenance of synaptic junctions. It triggers the de novo formation of presynaptic structures and may be involved in specification of excitatory synapses.