- ￥1200 - 3520
- 近岸蛋白(Novoprotein)
- 中国
- C692
- 2026年01月11日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Recombinant Mouse Macrophage metalloelastase/MMP12
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ￥3520.0 |
| 规格： | 500ug | 产品价格： | 询价 |
| 规格： | 1mg | 产品价格： | 询价 |
Recombinant Mouse Macrophage metalloelastase/MMP12 (C692)
产品说明(Description)
Recombinant Mouse Matrix Metalloproteinase 12 is produced by our Mammalian expression system and the target gene encoding Ala29-Cys473 is expressed with a 6His tag at the C-terminus.
Accession #: P34960
Known as: Macrophage metalloelastase;MME;Matrix metalloproteinase-12;MMP-12;Mmp12;Mme; Mmel
制剂（Formulation）
Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
Note: The proenzyme needs to be activated b.
质量控制（Quality Control）
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景（Background）
Matrix metalloproteinase-12（MMP12） is a secreted protein.It contains 4 hemopexin repeats and belongs to the peptidase M10A family. MMP12 may be involved in tissue injury and remodeling and have significant elastolytic activity. It can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
电泳(SDS-PAGE)
参考文献(Reference)
1、Design, synthesis and evaluation of 6-oxo-1, 6-dihydropyrimidine-2,5-dicarboxamide derivatives as MMP 13 inhibitors. LU Hai-bin. et al. Chem. Res. Chin. Univ. 2013.
FOR RESEARCH USE ONLY
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文献和实验Peritoneal Macrophage Preparation
OUTLINE This modification of peritoneal macrophage preparation is designed for a subsequent thrombophagocytosis test. PROTOCOL Sacrifice a mouse (use dry ice, CO2) Sink a mouse in the 5 water solution of Dettol
Macrophage Depletion by Clodronate
for no more than 7-10 days inject 200 µl /mouse, i.v. SOLUTIONS cholisterol solution = cholisterol, 8 mg + chloroform, 10 ml phosphatidilcholine (Sigma) = chloroform solution of phosphatidilcholine, 100 mg/ml clodronate solution
MACROPHAGE EXTRACTION: INTRAPERITONEAL LAVAGE
for anesthetizing 70 Ethanol for spraying Instrumentation, autoclaved: 2 surgical scissors, forceps, curled fine forceps 50ml conical tubes, sterile (one for each mouse genotype) Macrophage media: 20 FBS in DMEM high-glucose
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