Recombinant Human FEN-1/Flap endonuclease 1 (C140)
产品说明(Description)
Recombinant Human Flap Endonuclease 1 is produced by our E.coli expression system and the target gene encoding Met1-Lys380 is expressed. Accession #: P39748 Known as: Flap Endonuclease 1; FEN-1; DNase IV; Flap Structure-Specific Endonuclease 1; Maturation Factor 1; MF1; hFEN-1; FEN1; RAD2
制剂(Formulation) Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.
质量控制(Quality Control)
Purity: Greater than 90% as determined by reducing SDS-PAGE. Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping) The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
保存(Storage) Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
背景(Background) Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
电泳(SDS-PAGE)
参考文献(References) 1、EP300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis. He Huang. et al.Molecular Cell. 2018.