Recombinant Human EFNA4 (C-Fc-6His) (C480)
产品说明(Description)
Recombinant Human Ephrin-A4 is produced by our Mammalian expression system and the target gene encoding Leu26-Gly171 is expressed with a Fc, 6His tag at the C-terminus.
Accession #: P52798
Known as: Ephrin-A4; EPH-Related Receptor Tyrosine Kinase Ligand 4; LERK-4; EFNA4; EPLG4; LERK4
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
质量控制(Quality Control)
Bioactivity: |
Immobilized Human EphA7-His (Cat#C519) at 2μg/ml (100 μl/well) can bind Human EFNA4-Fc-His (Cat#C480).The ED50 of Human EFNA4-Fc-6His (Cat#C480) is 1.52 ug/ml. |
Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin: |
Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Ephrin-A4 is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. Ephrin-A4 consists of approximately 125 amino acids and includes four invariant cysteines, It has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. Ephrin-A4 binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. It may play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY