Recombinant Human CYB5B/Cytochrome b5 type B (CJ36)
产品说明(Description)
Recombinant Human Cytochrome b5 type B is produced by our Mammalian expression system and the target gene encoding Lys12-Cys118 is expressed with a 6His tag at the C-terminus.
Accession #: AAH04373.1
Known as: Cytochrome b5 outer mitochondrial membrane isoform;OMB5
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Cytochrome b5 type B (CYB5B) is a membrane of the cytochrome b5 family. It contains 1 cytochrome b5 heme-binding domain. Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. In the mitochondrion of eukaryotes and in aerobic prokaryotes, cytochrome b is a component of respiratory chain complex III also known as the bc1 complex or ubiquinol-cytochrome c reductase.