Recombinant Human B3GNT1/β-1,3-N-acetylglucosaminyltransferase 1 (C963)
产品说明(Description)
Recombinant Human N-Acetyllactosaminide beta-1,3-N-Acetylglucosaminyltransferase is produced by our Mammalian expression system and the target gene encoding Asp43-Cys415 is expressed with a 6His tag at the C-terminus.
Accession #: O43505
Known as: N-Acetyllactosaminide Beta-1;3-N-Acetylglucosaminyltransferase; I-Beta-1;3-N-Acetylglucosaminyltransferase; iGnT; Poly-N-Acetyllactosamine Extension Enzyme; UDP-GlcNAc:BetaGal Beta-1;3-N-Acetylglucosaminyltransferase 1; B3GNT1; B3GNT6
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM Tris HCl, 150mM NaCl, pH7.4
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
N-Acetyllactosaminide β-1,3-N-Acetylglucosaminyltransferase (B3GNT1) is a member of the β-1,3-N-Acetylglucosaminyltransferase family. B3GNT1 is a single-pass type II membrane protein and widely expressed in many tissues. B3GNT1 can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. B3GNT1 is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. It can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY