Recombinant Mouse IL-2

Recombinant Mouse IL-2  (CK24)

 

产品说明(Description)

Recombinant Mouse Interleukin-2 is produced by our E.coli expression system and the target gene encoding Ala21-Gln169 is expressed. 

Accession #: P04351

Known as: aldesleukin; interleukin 2; interleukin-2; IL-2; IL2; T-cell growth facter; T cell growth factor; TCGF 

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM Sodium Citrate,  0.2%Tween 80, pH 3.0.

 

质量控制(Quality Control) 

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity:   Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.

                       The specific activity of Recombinant Mouse IL-2 is ≥1×10⁷ IU/mg.

 

Bioactivity-BLI:
Loaded Human IL-2RA-Fc(Cat#CJ78) on Protein A Biosensor, can bind Mouse IL-2(Cat#CK24) with an affinity constant of 0.17 uM as determined in BLI assay.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis[J]. Chang‐Sheng Zhan. et al. FASEB Journal. 2020.

 

FOR RESEARCH USE ONLY