Recombinant Human IL-2

Recombinant Human IL-2  (C013)

产品说明(Description)

Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Ala21-Thr153 is expressed. 

Accession #: P60568

Known as: Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 10mM sodium citrate, pH 4.0.

 

质量控制(Quality Control)

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:     Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

 

Bioactivity:		Bioactivity-BLI:
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The specific activity of Recombinant Human IL-2 is ≥1×107 IU/mg.		Loaded Human IL-2RB-Fc(Cat#CJ82) on Protein A Biosensor, can bind Human IL-2(Cat#C013) with an affinity constant of 0.29 uM as determined in BLI assay.
Bioactivity-BLI:
Loaded Human IL-2RA-Fc(Cat#CJ78) on Protein A Biosensor, can bind Human IL-2(Cat#C013) with an affinity constant of 17.0 nM as determined in BLI assay.		Loaded Human IL-2RB-His(Cat#CJ81) on HIS1K Biosensor, can bind Human IL-2(Cat#C013) with an affinity constant of 0.27 uM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Interleukin-2(IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system，belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation.  IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response.  IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

A new immunotherapy strategy targeted CD30 in peripheral T-cell lymphomas: CAR-modified T-cell therapy based on CD30 mAb[J]. Yang Wu. et al. Cancer Gene Therapy. 2021.

MUC1-Tn-targeting chimeric antigen receptor-modified Vγ9Vδ2 T cells with enhanced antigen-specific anti-tumor activity[J]. Xiaochen Zhai. et al. American Journal of Cancer Research. 2021.

Cancer cell-derived exosomal circUHRF1 induces natural killer cell exhaustion and may cause resistance to anti-PD1 therapy in hepatocellular carcinoma[J]. Peng-Fei Zhang. et al. Molecular Cancer.2020.

MicroRNA124-IL6R Mediates the Effect of Nicotine in Inflammatory Bowel Disease by Shifting Th1/Th2 Balance Toward Th1[J]. Zhen Qin. et al. Frontiers in Immunology. 2021.

MxB impedes the NUP358-mediated HIV-1 pre-integration complex nuclear import and viral replication cooperatively with CPSF6[J]. Linlin Xie. et al. Retrovirology. 2020.

Relationships Among Circulating TNF-α, Skeletal Muscle and Peripheral CD8+ T Cells During Cachexia in Gastric Cancer[J]. Rui Zhao. et al. Cancer Biology. 2020.

HIV-2/SIV Vpx targets a novel functional domain of STING to selectively inhibit cGAS–STING-mediated NF-κB signalling[J]. Jiaming Su. et al. Nature Microbiology. 2019.

LILRB4 ITIMs mediate the T cell suppression and infiltration of acute myeloid leukemia cells[J]. Zunling Li. et al. Cellular & Molecular Immunology. 2019.

KrasG12D mutation contributes to regulatory T cell conversion through activation of the MEK/ERK pathway in pancreatic cancer[J]. He Cheng. et al. Cancer Letters. 2019.

MUC16 C terminal-induced secretion of tumor-derived IL-6 contributes to tumor-associated Treg enrichment in pancreatic cancer[J]. Kun Fan. et al. Cancer Letters. 2018.

 

 

FOR RESEARCH USE ONLY