Recombinant Mouse IL-1F1/IL-1

Recombinant Mouse IL-1F1/IL-1 alpha  (C041)

 

产品说明(Description)

Recombinant Mouse Interleukin-1 alpha is produced by our E.coli expression system and the target gene encoding Ser115-Ser270 is expressed. 

Accession #: P01582

Known as: Interleukin-1 Alpha; IL-1 Alpha; Il1a

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 50mM Tris-Hcl, 200mM NaCl, pH 8.0.

 

质量控制（Quality Control） 

Bioactivity*   Measured by the dose-dependent stimulation of mouse D10S cells.

                       The ED50 for this effect is less than 0.01 ng/ml.

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY