Recombinant Mouse RANK L

Recombinant Mouse RANK L (CR06)

产品说明(Description)

Recombinant Mouse TNF Ligand Superfamily Member 11 is produced by our E.coli expression system and the target gene encoding Lys158-Asp316 is expressed. 

Accession #: O35235

Known as: TNF Ligand Superfamily Member 11; Tumor necrosis factor ligand superfamily member 11; Tnfsf11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD254

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

 

质量控制(Quality Control) 

Purity:         Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:  Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such as thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Parathyroid Hormone Derivative with Reduced Osteoclastic Activity Promoted Bone Regeneration via Synergistic Bone Remodeling and Angiogenesis[J]. Jinghuan Huang. et al. Small. 2020.

Corilagin suppresses RANKL‐induced osteoclastogenesis and inhibits oestrogen deficiency‐induced bone loss via the NF‐κB and PI3K/AKT signalling pathways[J]. Jinwei Lu. et al. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2020.

The extract of Trachelospermum jasminoides (Lindl.) Lem. vines inhibits osteoclast differentiation through the NF-κB, MAPK and AKT signaling pathways[J]. Tao Jiang. et al. Biomedicine & Pharmacotherapy. 2020.

IGFBP7 acts as a negative regulator of RANKL-induced osteoclastogenesis and oestrogen deficiency-induced bone loss[J]. Chenyi Ye. et al. Cell Proliferation. 2019.

Lymphatic endothelial cells produce M-CSF causing massive bone loss in mice. Wensheng Wang. et al. Journal of Bone and Mineral Research. 2017.

The prevention of latanoprost on osteoclastgenesis in vitro and lipopolysaccharide‐induced murine calvaria osteolysis in vivo. Xing Xu. et al. Journal of Cellular Biochemistry. 2017.

Low-Dose IL-34 Has No Effect on Osteoclastogenesis But Promotes Osteogenesis of Hbmscs Partly Via Activation of The PI3K/AKT And ERK Signaling Pathways [J]. Jianxiang Xu. et al. Research square. 2020.

 

 

FOR RESEARCH USE ONLY