Recombinant Human IL-3RA/CD123 (CU77)
产品说明(Description)
Recombinant Human Interleukin-3 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Thr19-Arg305 is expressed with a Fc tag at the C-terminus.
Accession #: P26951
Known as: CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL3R; IL-3Ra; IL-3R-alpha; interleukin-3 receptor subunit alpha
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity-BLI:
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Loaded Human IL-3RA-Fc(Cat#CU77) on Protein A Biosensor, can bind Human IL-3(Cat#CF63) with an affinity constant of 3.89uM as determined in BLI assay. |
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Loaded Human IL-3RA-Fc(Cat#CU77) on Protein A Biosensor, can bind Human IL-3(Cat#CF63) with an affinity constant of 3.89uM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
CD123, also known as Interleukin-3 receptor subunit alpha, belongs to the type I cytokine receptor family. In mouse, there are two classes of high-affinity IL3 receptors. One contains an IL3-specific beta subunit and the other contains the beta subunit also shared by high-affinity IL5 and GM-CSF receptors. CD123 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. CD123 is a heterodimer consisting of an alpha and a beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY