相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃保存,有效期一年;切勿冻结!
- 保质期:
4℃保存,有效期一年;切勿冻结!
- 英文名:
Nickle-coupled Agarose Affinity Matrix
- 库存:
大量
- 供应商:
上海康朗生物科技有限公司
- 规格:
ml/盒
金属镍琼脂糖亲和介质是络合金属镍离子(Ni)的4%浓度的交联珠状琼脂糖介质,可用于结合和分离富含组氨酸(Histidine)等残基的蛋白质分子。介质的蛋白质结合容量因蛋白不同而有差异,一般每ml介质可结合数百微克或数毫克蛋白介质可再生后重复使用数次而不丧失载量。
用途
· 带有His标签的蛋白纯化
特点
· 高结合量:每毫升基质可结合高达数毫克的融合蛋白*
· 高度灵活:可填装任意品牌的柱子
储存条件
4℃保存,有效期一年;切勿冻结!
主要成分
50% Ni-Agarose亲和基质悬液,PBS,20%乙醇
干扰成分参考表
| 可能对蛋白结合有影响的缓冲液成分 | 使用浓度应低于 |
| Tris, HEPES, MOPS | 100 mM |
| EDTA, EGTA | 1 mM |
| 2-mercaptoethanol | 20 mM |
| DTT, DTE | 1 mM |
| Triton, Tween, NP-40 | 2% |
| SDS, Sarkosyl | 0.3% |
*注:基质的蛋白结合容量因蛋白不同而有差异,通常每毫升基质可结合数百微克至数毫克的融合蛋白。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Orientation of the Agarose Matrix by Pulsed Electric Fields
DNA molecules less than ~20 kilobase (kb) pairs in size are efficiently fractionated by unidirectional electrophoresis in agarose gels (1 ). The variation of DNA mobility with gel concentration can be described by the Ogston mechanism
On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis
The coupling of on-column derivatization of small molecules to affinity capillary electrophoresis (ACE) has only been realized during the past 5 yr. In this technique, multiple zones of reagent(s) and ligand or receptor are injected
Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
attempted affinity methods. Our laboratory has used Reactive Red-agarose in the purification of m-and μ-calpain from bovine heart and other tissues for many years (1 ,2 ). However, this method has not achieved widespread use due to reported variability
技术资料暂无技术资料 索取技术资料





