Recombinant Human Mutated Vime

Source

E. coli­-derived
Ser2­-Glu466, G16R, G59R, with a C-­terminal 6­-His tag  

Accession #

P08670

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

54.7 kDa

SDS PAGE

56 kDa, reducing conditions.

Activity

Correct folding was measured by circular dichroism spectrum

Endotoxin Level

< 0.10 EU per 1 μg of the protein by the LAL method.

Purity

> 98%, by SDS PAGE under reducing conditions and visualized by Coomassie Blue stain at 2 μg per lane.

Formulation

Supplied as a 0.2 μm filtered solution in 5 mM Tris-HCl, 5mM BME, pH=8.5

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Avoid repeated freeze-thaw cycles.
•6 months from date of receipt, -80 ◦C as supplied.
•3 months, -80 ◦C under sterile conditions after opening.

ACTIVITY ASSAY PROTOCOL DATA

SDS PAGE





Bioactivity

0.2 mg/mL of BioRobust Vimentin product was evaluated using circular dichroism spectrometer. The spectrum shown below indicates that the protein is correctly folded with extensive α-helixes.




BACKGROUND

Vimentin is a 54 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-­2). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled­-coil regions separated by linkers (1, 2). Vimentin with G16R and G59R double mutations when citrullinated by PAD (peptidyl arginine deaminase) could be used as an antigen to detect autoimmune antibodies in Rheumatoid Arthritis diagnosis (3).

Entrez Gene IDs:

3417 (Human); 15926 (Mouse); 24479 (Rat)