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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
美国
- 亚型：
10ml
- 保存条件：
RT
- 克隆性：
多克隆
- 浓度：
20 mM Citrate pH 3.0 (Sterile)
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文献和实验Microwave citrate Pretreatment of Paraffin Sections
or Tek® staining dish and fill container with 10 mM citrate bufferb, c , pH 6.0.
Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
1. Dissect embryos and place each yolk sac in a microfuge tube (can be frozen at -20°C prior to extraction). Original method cited for mouse toes, or 2 mm tail, Reagents: A) 25 mM NaOH / 0.2 mM EDTA. The pH is about 12. B) 40 mM Tris HCl
Immobilized pH gradient (IPG) as first dimension
strips were prepared by Pharmacia LKB Biotechnology AB and are commercially available. The strips were 3 mm wide and 180 mm long. In addition, the geometry of the immobilized pH gradient strips has been changed to allow the use of large sample application
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