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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
美国
- 亚型:
15ml
- 形态:
ELISA
- 保存条件:
-20C
- 克隆性:
多克隆
- 浓度:
Cell Lysis Buffer (10X)
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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer Day -2: o Start a 5ml overnight culture from a single colony. Day -1: o Start a larger overnight culture using part of the 5ml culture (subculture). Day
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
cells twice with PBS pouring excess off into waste beaker Carefully soak up any extra PBS with a Kimwipe Add 150ul of RIPA lysis buffer to the culture dish Use cell scraper to scrape cells from the bottom of the dish Pass cell
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